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A turning disk confocal microscope simultaneously illuminates of several excitation light locations and you can detects the new delivered fluorescence of per location inside parallel. A turning confocal disk scans excitation places if you are related pinholes on the the newest advanced imaging jet slow down the away from interest fluorescence. The brand new spatial shipment of your own fluorescence laws will be detected because of the a cam (Figure 2). As the shown on the strategy away from pinhole number in the Shape 2, the length ranging from pinholes for the confocal drive is actually adequate to avoid laws crosstalk anywhere between pinholes. What’s more, it understands high speed imaging as quickly as 200 frames per second with rotating disk confocaⅼ fluorescence microscopy. But not, the true quality is generally exactly like compared to a widefield fluorescence microscope since the higher-frequency signals are weak.
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